Carbohydrate Polymers 88 373-381. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Journal of Physical Chemistry, 58(11), 953-957. ; Talsma, H.; Hennink, W.E. Fluorescent dextran Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. WebFITC Products. Dilute solution properties and degree of chain branching Solution properties of branched dextran. These studies were carried out at the Hisko (Gunpo, Korea). ; Veldhuis, T.F.J. The exponent a is a function of polymer geometry, and varies from 0.5 to 2 and exponent is dimensionless. Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. The https:// ensures that you are connecting to the To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. (2003). In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). ; Hogan, M.P. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. dextran hydrodynamic radius (2003). The micropore formed at the SC through to the epidermis, enabling drug delivery. volume24, Articlenumber:22 (2020) The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. II. Guner, A.; Kibarer, G. (2001). J Appl Toxicol. Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. At 25C both values of a and kM-H are within the range reported by [3, 8, 16]. Epub 2012 May 28. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. Indo AM J Pharm. 2004;25(9):155967. Learn More. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. Format). The .gov means its official. Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. The formula is verified for different systems of polymer-solvent and the values are in accord with those obtained by extrapolation. Degrees of long-chain branching in dextrans. Ghimici, Luminita; Nichifora, Marieta; Eich, Andreas; Wolf, Bernhard A. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. Unperturbed dimensions and theta temperature of dextran in aqueous solutions. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. @ Nilimesh See attached. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. A solvent at this temperature is called a theta solvent [53]. Die Makromolekulare Chemie, 148(1), 229-238. Journal of American Chemical Society, 76 (20), 5041-5052. 2015;23(3):2239. Thermo Fisher Scientific. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. Progr. 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. SEC of mono-carboxymethyl cellulose (CMC) in a wide range of pH, MarkHouwink constants. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Learn More. 2012;87(6):1728. The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. All authors have consented to the submission of this manuscript for publication. Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. Int J Food Sci Nutr. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. 1984;17(6):11703. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. Correspondence to WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Manage cookies/Do not sell my data we use in the preference centre. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. 2013;43(4):287303. Careers. (2001). Journal of American Chemical Society, 64(11), 2716-2718. 2013, Article ID 360239, pp. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. ; Garca de , J. (2008). Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. Chem. The mechanism of the dextran-induced red blood cell Aggregation. (1990). The most widely used method for the characterization of macromolecules is the capillary viscometry, as it is a simple and economic method. Webof overall macromolecular geometry and hydrodynamic draining. Drug Deliv Syst. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. (2012). Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in TEWL measurements were performed before and 30min after microporation. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. Arond L.H. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Carbohydrate Polymers, 74, 372-378. A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. Qi P, Caoa M, Songa L, Chena C, Liua M, Li N, Wua D, Penga J, Hub G, Zhao J. 2005;304(1):27486. Accessibility Pharmaceut Sci Tech. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. Microporation was performed 2, 5, and 10 times on each sample. Pharmacol Pharm. Privacy Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. Article TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. Materiaux 1-5. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. Food Funct. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. European Polymer Journal, 37, 619-622. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. Not for use in diagnostic procedures. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. Small Intestinal Permeability and Gut-Transit Time Determined Radius Carbohydrate Polymers, 39, 377-380. (2007). ; Rjumtsev, E.I. (2003). Nanoparticles in magnetic resonance imaging: from simple to dual a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Nair SS. The characteristic Rg/Rh value for a globular protein is ~0.775, which means that Rg is smaller than Rh. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. Drug Deliv. where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). Article Herwadkar A, Banga AK. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). 2010;4(1):117. Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. European Polymer Journal, 43, 1744-1753. Colloid and Polymer Science, 286, 1223-1231. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. Journal of Applied Polymer Science, 13, 2031-2032. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Macromolecules, 29, 2265-2268. B 113, 8020-8025. Front Microbiol. 2015;32(5):170413. The micropores of the human cadaver skin were regenerated according to the time. eCollection 2018 Feb. Molecules. Chen Rong Huei; Min Lang Tsai (1998). The Mark-Houwink. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). Table 2 compares the properties of nanoparticles coated with one or other polymer. Springer Editions. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. CAS WebTry out PMC Labs and tell us what you think. (2008). Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. 2009;6(4):34354. Dextran Figure5 shows the cumulative amount of the FITC-dextran (M.W. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. 2016;9706:97061E6. MeSH The slope is a value and intercept is equal to the ln kMH value [47]. The correct way to interpret what Mark-Houwink parameters is determined graphically ln [] versus ln Mw, where Mw is the molecular weight provided by manufacturer, and calculate the percentage relative error (%RE) respect to Mw. The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). Nordmeier, Eckliard (1993). ; Ghosh, T.K. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. Intrinsic viscosity molecular weight relationship. dextran Biophysical Journal, 27, 177-181. 4K, b M.W. In vivo iontophoretic delivery of salmon calcitonin across microporated skin. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Structural and biocompatibility properties of dextran from This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Chem. Ann Surg Oncol. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. Carbohydrate Polymers, 36, 319-326. International Journal of Pharmaceutics, 277, 99-104. Chem. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". 2012;101(8):28619. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. Castellanos Gil, E. E.; Iraizoz Colarte, A.; El Ghzaoui, A.; Durand, D.; Delarbre, J.L. Macromolecules, 33, 5730-5739. The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. Radiofrequency ablation of malignant liver tumors. Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th Global hydrodynamic analysis of the molecular flexibility of galactomannans. Measurements were made with a Ubbelohde C type suspended level viscometer [57] (IVA, Argentina); with a diameter capillary of 0.81mm and water draining time of 35.89s at 20C, with viscometer constant of 0.027883 mm2/s [58]. We observe that this maximum radius is below Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. It is well tolerated on parenteral infusion by most people but a very low incidence Structural and biocompatibility properties of dextran from These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). Int J Pharm Investig. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. Carbohydrate Research, 340, 811-826. Synthesis and characterization of blue dextrans. 2000;3(9):31826. GPC Symp. Liu Zonghua; Yanpeng Jiao; Yifei Wang; Changren Zhou; Ziyong Zhang (2008). Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. Bohdanecky, Miloslav (1996). Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim.
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